A total of 96 gamblers played a slot machine simulator with and without sound being paired with reinforcement. In the current study, we investigate the role that sound plays in psychophysical responses to slot machine play. It is the use of sound for positive reinforcement in particular that we believe influences the player. Sound plays an important role in reinforcement, and thus on arousal level and stress response of players. We believe that sound is one of these key contributing factors. The contributing factors to these effects remain under-explored. Slot machine wins and losses have distinctive, measurable, physiological effects on players.